Summary

Write yourself a full summary of the procedure before you do it. You’ll want a quick reference that you full own and understand on the day of your brew so that you’re not stumbling around reading the website to figure out what’s next.

Here’s an example copied from HRT Cat.

Example

Do not use this, make your own. There may be errors in this.

• Clear work area
• Sweep and mop
• Hang plastic sheets
• Install and turn on HEPA air purifier
• Sanitize work surface
• Clean floor once more
• Begin wearing shoe covers and garbs in clean room
• Preheat oven to 250°C
• Wash, rinse, and wrap items for depyrogenation
• Put in oven once pre-heated, set timer for 2 hours
• Prepare autoclave with distilled water
• Wash, rinse, and wrap items for sterilization
• Autoclave for 1 hour.
• Wipe down everything else with IPA
• Setup laminar flow hood, leave on
• Remove items from oven and autoclave, stage in clean room
• Inside flow hood, mix in beaker:
  • ____mL MCT oil
  • ____mL Benzyl Alcohol
  • ____mL Benzyl Benzoate
  • ____mg Estradiol Enanthate
• Filter into closed 100mL vials
• Dispense into vial, place stopper immediately. Repeat.
• Cap.
• Bubble point test
  • Listed bubble point: ____psi
  • Today’s air pressure: ____psi
  • Starting air volume: ____mL
  • Actual bubble point volume: ____mL
  • Actual bubble point (calculator ): ____psi
• Full visual inspection
• Make a record
• Label vials